Composite

Part:BBa_K094111

Designed by: Li Xinran   Group: iGEM08_iHKU   (2008-10-13)


pTetR-rbs-cheY*

This part uses BBa_J61003 as a backbone and cheY* as the inserted gene. cheY is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part lacks a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1
    Illegal EcoRI site found at 2058
    Illegal XbaI site found at 2292
    Illegal PstI site found at 2564
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2058
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal PstI site found at 2564
    Illegal NotI site found at 9
    Illegal NotI site found at 2064
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2058
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2
    Illegal EcoRI site found at 2058
    Illegal XbaI site found at 2292
    Illegal PstI site found at 2564
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2058
    Illegal XbaI site found at 2292
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal PstI site found at 2564
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1097


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Categories
Parameters
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